Journal
MECHANISMS OF AGEING AND DEVELOPMENT
Volume 190, Issue -, Pages -Publisher
ELSEVIER IRELAND LTD
DOI: 10.1016/j.mad.2020.111318
Keywords
oxidative stress; cigarette smoke; epidermal thinning; collagen degradation
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Funding
- Aktion D. Swarovski KG 2018 [281886]
- Erasmus + fellowship
- FWF [P 31582-B26]
- Doctoral Programme Ageing and Regeneration (DP AGE_REG), University of Innsbruck
- Erwin Schroedinger Abroad Fellowship [J4205-B27]
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Accumulation of senescent cells promotes the development of age-related pathologies and deterioration. In human skin, senescent cells potentially impair structure and function by secreting a mixture of signaling molecules and proteases that influence neighboring cells and degrade extracellular matrix components, such as elastin and collagen. One of the key underlying mechanisms of senescence and extrinsic skin aging is the increase of intracellular reactive oxygen species and resulting oxidative stress. Tert-butyl hydroperoxide (tBHP) is a known inducer of oxidative stress and cellular damage, acting at least in part by depleting the antioxidant glutathione. Here, we provide a detailed characterization of tBHP-induced senescence in human dermal fibroblasts in monolayer culture. In addition, results obtained with more physiological experimental models revealed that tBHP treated 3D reconstructed skin and ex vivo skin developed signs of chronic tissue damage, displaying reduced epidermal thickness and collagen fiber thinning. We, therefore, propose that tBHP treatment can be used as a model to study the effects of extrinsic skin aging, focusing mainly on the influence of environmental pollution.
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