Journal
LWT-FOOD SCIENCE AND TECHNOLOGY
Volume 137, Issue -, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.lwt.2020.110436
Keywords
High hydrostatic pressure (HHP); Whey protein isolate (WPI); Refolding; Techno-functional properties; Denaturing gel electrophoresis (SDS-PAGE)
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Funding
- University of Salerno (Dept. of Industrial Engineering)
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This study assessed the effect of storage time on the structure and functionality of high-pressure-processed whey protein isolate (WPI), and found that the optimal treatment conditions were 400 MPa and 15 minutes for maximum unfolding and the highest hydrolysis yield. High-pressure significantly promoted the hydrolysis performances of selected enzymes towards WPI, particularly with a clear synergistic effect of alpha-chymotrypsin and bromelain combination.
This work assessed the effect of storage time on the structure and functionality of HHP-treated whey protein isolate (WPI). Different pressures (100-600 MPa) and treatment times (15-30 min) were applied to aqueous WPI dispersions (5% w/v). The induced degree of unfolding was evaluated to select optimal HHP treatment conditions of WPI before storage at 4 degrees C. Conformational and techno-functional properties of untreated and optimally HHP-treated WPI samples were determined by UV-Vis and IR spectroscopy, foaming capacity, and interfacial tension measurements, respectively. Further tests of HHP-assisted hydrolysis of WPI were performed by alpha-chymotrypsin, bromelain, or their mixture (1:1 w/w), with the degree of hydrolysis (DH%) and electrophoretic patterns analyzed. The maximum unfolding degree was detected after a treatment of 400 MPa and 15 min and, at these processing conditions, no aggregation occurred. However, the structural changes achieved upon HHP were gradually lost during storage through a first-order refolding process (k(REF.) = 0.031 h(-1)), with restoring of native functionality. Hydrolysis performances of selected enzymes towards WPI were significantly promoted by high-pressure. Interestingly, a clear synergistic effect of alpha-chymotrypsin and bromelain combination on the WPI hydrolysis yield was detected, which resulted in the highest protein rupture (DH = 17%).
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