Journal
LWT-FOOD SCIENCE AND TECHNOLOGY
Volume 131, Issue -, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.lwt.2020.109748
Keywords
Citrus honey; Floral nectar; Chemical composition; EESI-HRMS
Categories
Funding
- National Natural Science Foundation of China [31772067]
- Department of Science and Technology of Jiangxi Province [20165BCB18005, 20171BCB24002]
- Jiangxi Agriculture Research System
- Research Project of State Key Laboratory of Food Science and Technology [SKLF-ZZB-201918]
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Analyzing the chemical composition of nectar and honey will contribute to honey traceability, which has become a research hotspot in recent years. However, the rapid, green, and facile analytical methods without tedious chemical pretreatment remain to be developed. Herein, the extractive electrospray ionization high resolution-mass spectrometry (EESI-HRMS) was used to analyze the polyphenols and amino acids composition of the nectars and honeys from three Citrus species, and high-performance liquid chromatography coupled to ultra-violet detector (HPLC-UV) was used to validate the results. 12 polyphenols and 8 amino acids were identified by EESI-HRMS, whilst 9 polyphenols were quantified by HPLC-UV. Principal component analysis (PCA) revealed that the chemical composition of Citrus nectars was similar, and so do honeys, but the nectar and its corresponding honey were chemically differentiated. Accordingly, EESI-HRMS is a reliable analytical method for the analysis of samples with limited sources and complex composition, which contributes to the honey traceability.
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