4.7 Article

Dietary stevioside supplementation increases feed intake by altering the hypothalamic transcriptome profile and gut microbiota in broiler chickens

Journal

JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
Volume 101, Issue 5, Pages 2156-2167

Publisher

WILEY
DOI: 10.1002/jsfa.10838

Keywords

stevioside; feed intake; hypothalamus; gut microbiota; broiler chicken

Funding

  1. Jiangsu Agriculture Science and Technology Innovation Fund (JASTIF) [CX(18)2002]

Ask authors/readers for more resources

The study revealed that dietary supplementation of stevioside can promote feed intake in chickens through regulating the hypothalamic neuroactive ligand-receptor interaction pathway and altering the composition of gut microbiota.
BACKGROUND Stevioside (STE) is a widely used sweetener. Despite the fact that chickens are insensitive to sweetness, dietary STE supplementation could increase the feed intake of broiler chickens. Stevioside might regulate the feeding behavior through functional mechanisms other than its high-potency sweetness. The present study was aimed to elucidate the potential sweetness-independent mechanism of an STE-induced orexigenic effect using the broiler chicken and considering the hypothalamic transcriptome profile and gut microbiome. RESULTS The analysis of RNA-Seq identified 398 differently expressed genes (160 up-regulated and 238 down-regulated) in the hypothalamus of the STE-supplemented group compared with the control group. Cluster analysis revealed several appetite-related genes were differentially expressed, includingNPY,NPY5R,TSHB,NMU,TPH2, andDDC. The analysis of 16S rRNA sequencing data also indicated that dietary STE supplementation increased the relative abundance ofLactobacillales,Bacilli,Lactobacillus, andLactobacillaceae. Meanwhile, the proportion ofRuminococcaceae,Lachnospiraceae,Clostridia, andClostridialeswas decreased after dietary supplementation with STE. CONCLUSION Dietary STE supplementation promoted feed intake through the regulation of the hypothalamic neuroactive ligand-receptor interaction pathway and the alteration of intestinal microbiota composition. This study provides valuable information about the sweetness-independent mechanism of the STE-induced orexigenic effect using the broiler chicken (which is insensitive to sweetness) as the animal model. (c) 2020 Society of Chemical Industry

Authors

I am an author on this paper
Click your name to claim this paper and add it to your profile.

Reviews

Primary Rating

4.7
Not enough ratings

Secondary Ratings

Novelty
-
Significance
-
Scientific rigor
-
Rate this paper

Recommended

No Data Available
No Data Available