A novel ionic liquid based electrochemical sensor for detection of pyrazinamide

In this work, we report electrochemical detection of pyrazinamide at the surface of a glassy carbon electrode fabricated with a sensing nanocomposite comprising silver nanoparticles, 1-ethyl-3-methylimidazolium tetrafluoroborate ([Emim][BF4]) ionic liquid, and reduced graphene oxide. The electrochemical reaction of the pyrazinamide at the fabricated electrode was measured using cyclic and differential pulse voltammetric methods. Ionic liquid-reduced graphene oxide-silver nanoparticles were successfully synthesized and characterized by transmission electron microscope and Fourier transform infrared spectroscopy, respectively. The synthesized silver nanoparticles were spherical in shape with an average size of similar to 30 nm. The fabricated electrode exhibits excellent electrocatalytic utility with favourable electrochemical parameters for the reduction of pyrazinamide in acidic media, with optimized pH of 6.0 at an operational potential of - 0.82 V (vs. SCE). The transmission electron microscopy results for the fabricated surfaces demonstrated the formation of nanoscale structures, representing successful electrodeposition and electro-polymerization processes to modify the glassy carbon electrode surface. Moreover, these results showed a linear electrochemical response to pyrazinamide within the concentration in the interval ranging from 3 to 24 mu M, with the detection limit and limit of quantification of 0.0102 mu M and 0.3658 mu M. The fabricated sensor proved high selectivity and sensitivity for pyrazinamide, which made it possible to determine pyrazinamide in pharmaceutical formulations. [GRAPHICS] .

Automated summary

In this study, a sensing nanocomposite consisting of silver nanoparticles, ionic liquid, and reduced graphene oxide was successfully synthesized and used for the electrochemical detection of pyrazinamide. The fabricated electrode exhibited excellent electrocatalytic activity for the reduction of pyrazinamide in acidic media, with a linear electrochemical response and a detection limit of 0.0102 mu M.