Journal
JOURNAL OF RECEPTORS AND SIGNAL TRANSDUCTION
Volume 41, Issue 5, Pages 494-503Publisher
TAYLOR & FRANCIS LTD
DOI: 10.1080/10799893.2020.1828915
Keywords
Dilated cardiomyopathy; lncRNA AC061961; 2; endoplasmic reticulum stress; apoptosis; Wnt; β -catenin
Categories
Funding
- Natural Science Foundation of Jiangsu Province [BK20181119]
Ask authors/readers for more resources
The down-regulated lncRNA AC061961.2 in dilated cardiomyopathy (DCM) patients has a regulatory effect on DCM. Overexpression of AC061961.2 can inhibit endoplasmic reticulum stress-induced apoptosis in DCM rats and cardiomyocytes by activating the Wnt/beta-catenin pathway.
Down-regulated lncRNA AC061961.2 in dilated cardiomyopathy (DCM) patients was previous reported. Whether AC061961.2 has regulatory effect on DCM still need exploration. Here, we tried to investigate the effect of AC061961.2 on DCM. After DCM model rat was established through injecting Adriamycin, left ventricular end-diastolic diameter (LVEDD), left ventricular end-systolic diameter (LVESD), left ventricular ejection fraction (LVEF), and left ventricular fractional shortening (LVFS) were measured by echocardiography. Histopathological changes and apoptosis were detected by hematoxylin-eosin, Masson staining, and TUNEL. After cardiomyocytes were isolated and identified by immunofluorescence, DCM cell model was established by injecting adriamycin. After transfected with overexpressed-AC061961.2 plasmids, cell apoptosis was detected by flow cytometry. The expressions of AC061961.2, beta-catenin, Axin2, c-Myc, CRP78, CHOP, Caspase-3, Bcl-2, and Bax in cardiomyocytes and heart tissues were detected by RT-qPCR or western blot. LVEDD and LVESD were increased while LVEF and LVFS were decreased in DCM rats. The histopathological of heart tissues showed a typical sign of DCM. Apoptosis were increased in heart tissues of DCM rats. In DCM rats, the expressions of AC061961.2, beta-catenin, Axin2, c-Myc, and Bcl-2 were decreased, the expressions of CRP78, CHOP, Caspase-3, and Bax were increased. After the overexpression of AC061961.2, levels of beta-catenin, Axin2, c-Myc, and Bcl-2 were increased, while levels of CRP78, CHOP, Caspase-3, and Bax were decreased, compared with that in DCM cardiomyocytes. LncRNA AC061961.2 overexpression inhibited endoplasmic reticulum stress induced apoptosis in DCM rats and cardiomyocytes via activating Wnt/beta-catenin pathway.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available