Journal
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
Volume 190, Issue -, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.jpba.2020.113492
Keywords
Human blood serum; Polar pesticides; LC; QqQ; Glyphosate; Toxicity
Categories
Funding
- Plan Propio de Investigation of University of Almeria
- CAJAMAR
- Operational Program Funds Europeans of Regional Development of Andalusia (20142020) (FEDER)
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In this study a simple and rapid method was used for the determination of 5 polar pesticides and 3 metabolites, ethephon, 2-hydroxyethyl phosphonic acid (HEPA), fosetyl-aluminum, phosphonic acid, glyphosate, chlorate, glufosinate and 3-methylphosphinicopropionic acid (MPPA) in human blood serum. Acidified water (1% formic acid) and acetonitrile (1% formic acid) were used as extraction solvents and n-hexane was utilized as a clean-up step for lipid removal. The analytical method was based on liquid chromatography coupled to triple quadrupole tandem mass spectrometry (LC-QqQ-MSIMS). The proposed method was validated, obtaining recoveries ranging from 70 to 110% and precision values lower than 17% for all analytes. Limit of quantification was 0.01 mg/L for ethephon, fosetyl-aluminum, MPPA and HEPA, 0.025 mg/L for glyphosate, phosphonic acid and chlorate and 0.05 mg/L for glufosinate. This is the first time that an analytical method was developed for the determination of a high number of polar pesticides in human blood serum, allowing the simultaneous monitoring of these compounds in a complex matrix as human blood serum. (C) 2020 Elsevier B.V. All rights reserved.
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