4.5 Article

Ppp1r3d deficiency preferentially inhibits neuronal and cardiac Lafora body formation in a mouse model of the fatal epilepsy Lafora disease

Journal

JOURNAL OF NEUROCHEMISTRY
Volume 157, Issue 6, Pages 1897-1910

Publisher

WILEY
DOI: 10.1111/jnc.15176

Keywords

glycogen; glycogen synthase; glycogen targeting subunit; Lafora; laforin; PPP1R3D

Funding

  1. National Institute of Neurological Disorders and Stroke [P01 NS097197]

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This study investigates the impact of eliminating different PP1 subunits, such as PPP1R3D and PPP1R3C, on the formation of Lafora bodies and glycogen metabolism in the nervous system. The research reveals that PPP1R3C is the major isoform in most tissues, while PPP1R3D plays a significant role in neurons and cardiomyocytes. These findings contribute to a better understanding of brain glycogen metabolism and Lafora disease.
Mammalian glycogen chain lengths are subject to complex regulation, including by seven proteins (protein phosphatase-1 regulatory subunit 3, PPP1R3A through PPP1R3G) that target protein phosphatase-1 (PP1) to glycogen to activate the glycogen chain-elongating enzyme glycogen synthase and inactivate the chain-shortening glycogen phosphorylase. Lafora disease is a fatal neurodegenerative epilepsy caused by aggregates of long-chained, and as a result insoluble, glycogen, termed Lafora bodies (LBs). We previously eliminated PPP1R3C from a Lafora disease mouse model and studied the effect on LB formation. In the present work, we eliminate and study the effect of absent PPP1R3D. In the interim, brain cell type levels of all PPP1R3 genes have been published, and brain cell type localization of LBs clarified. Integrating these data we find that PPP1R3C is the major isoform in most tissues including brain. In the brain, PPP1R3C is expressed at 15-fold higher levels than PPP1R3D in astrocytes, the cell type where most LBs form. PPP1R3C deficiency eliminates similar to 90% of brain LBs. PPP1R3D is quantitatively a minor isoform, but possesses unique MAPK, CaMK2 and 14-3-3 binding domains and appears to have an important functional niche in murine neurons and cardiomyocytes. In neurons, it is expressed equally to PPP1R3C, and its deficiency eliminates similar to 50% of neuronal LBs. In heart, it is expressed at 25% of PPP1R3C where its deficiency eliminates similar to 90% of LBs. This work studies the role of a second (PPP1R3D) of seven PP1 subunits that regulate the structure of glycogen, toward better understanding of brain glycogen metabolism generally, and in Lafora disease.

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