Cholinergic transmission in C.elegans: Functions, diversity, and maturation of ACh-activated ion channels

Acetylcholine is an abundant neurotransmitter in all animals. Effects of acetylcholine are excitatory, inhibitory, or modulatory depending on the receptor and cell type. Research using the nematodeC.eleganshas made ground-breaking contributions to the mechanistic understanding of cholinergic transmission. Powerful genetic screens for behavioral mutants or for responses to pharmacological reagents identified the core cellular machinery for synaptic transmission. Pharmacological reagents that perturb acetylcholine-mediated processes led to the discovery and also uncovered the composition and regulators of acetylcholine-activated channels and receptors. From a combination of electrophysiological and molecular cellular studies, we have gained a profound understanding of cholinergic signaling at the levels of synapses, neural circuits, and animal behaviors. This review will begin with a historical overview, then cover in-depth current knowledge on acetylcholine-activated ionotropic receptors, mechanisms regulating their functional expression and their functions in regulating locomotion.

Automated summary

Acetylcholine is a neurotransmitter with excitatory, inhibitory, or modulatory effects depending on the receptor and cell type. Research using the nematode C. elegans has contributed to the understanding of cholinergic transmission. Genetic screens and pharmacological reagents have helped identify the core cellular machinery for synaptic transmission and regulate acetylcholine-activated channels and receptors.