Journal
JOURNAL OF MATERNAL-FETAL & NEONATAL MEDICINE
Volume 35, Issue 16, Pages 3209-3215Publisher
TAYLOR & FRANCIS LTD
DOI: 10.1080/14767058.2020.1817369
Keywords
Unexplained recurrent pregnancy loss; maternal-fetal interface; decidual natural killer cells; mitogen-activated protein kinase signal pathway
Categories
Funding
- National Natural Science Foundation of China [81270733]
- Beijing Natural Science Foundation [7132097]
Ask authors/readers for more resources
This study investigated the expression levels of TNF-alpha, IFN-gamma, IL-4, and IL-10 in dNK cells and found that the MAPK signal pathway is involved in the regulation of cytokine secretion by decidual NK cells at the maternal-fetal interface.
Objective This study aims to investigate the expression levels of TNF-alpha, IFN-gamma, IL-4, and IL-10 in dNK cells and determine whether or not the MAPK signal pathway is involved in the regulation of cytokine secretion by dNK cells at the maternal-fetal interface. Methods In this study, we collected decidua specimens from patients with apparently normal pregnant and unexplained recurrent pregnancy loss (URPL) and extracted dNK cells by enzymatic digestion. Then the expression of cytokines were analyzed by flow cytometry and Real-Time PCR respectively. Results The secretions of both IFN-gamma and TNF-alpha in dNK cells in URPL were significantly higher than those in normal pregnancy. Furthermore, p38/MAPK inhibitors can inhibit the secretion of four cytokines in normal pregnancy, while in URPL cases, p38/MAPK inhibitors only significantly inhibit the secretion of IL-4 and IFN-gamma. ERK inhibitors had no effect on the expression of all four cytokines and JNK/MAPK inhibitors varied on different cytokines. Conclusion URPL is associated with a NK1 cytokine profile. MAPK signaling pathway is involved in the regulation of cytokine secretion by decidual NK cells at maternal-fetal interface.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available