Journal
JOURNAL OF MANUFACTURING SCIENCE AND ENGINEERING-TRANSACTIONS OF THE ASME
Volume 143, Issue 1, Pages -Publisher
ASME
DOI: 10.1115/1.4048853
Keywords
additive manufacturing
Funding
- Air Force Office of Scientific Research (AFOSR) [FA9550-18-1-0233]
Ask authors/readers for more resources
This study experimentally investigates the effects of extrusion pressure and needle diameter on cell quantity in extrusion-based bioprinting of hydrogel-based bioink containing Chlamydomonas reinhardtii algae cells. The results show that, as the extrusion pressure increases or the needle diameter decreases, the quantity of algae cells in printed samples decreases.
Bioprinting is the fabrication of structures based on layer-by-layer deposition of biomaterials. Applications of bioprinting using plant or algae cells include the production of metabolites for use in pharmaceutical, cosmetic, and food industries. Reported studies regarding effects of extrusion pressure and needle diameter on cell viability in bioprinting have used animal cells. There are no reports regarding effects of extrusion pressure and needle diameter on cell viability using plant or algae cells. This paper fills this knowledge gap by reporting an experimental investigation on effects of extrusion pressure and needle diameter on cell quantity (an indicator of cell viability) in extrusion-based bioprinting of hydrogel-based bioink containing Chlamydomonas reinhardtii algae cells. Extrusion pressure levels used in this study were 3, 5, and 7 bar, and needle diameter levels were 200, 250, and 400 mu m. Algae cell quantity in printed samples was measured on the third day and sixth day post bioprinting. Results show that, when extrusion pressure increases or needle diameter decreases, algae cell quantity in printed samples decreases.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available