Journal
JOURNAL OF INORGANIC BIOCHEMISTRY
Volume 211, Issue -, Pages -Publisher
ELSEVIER SCIENCE INC
DOI: 10.1016/j.jinorgbio.2020.111194
Keywords
Quinazoline derivatives; Zn(II) complexes; Interaction with calf-thymus DNA; Plasmid DNA-photocleavage; Interaction with albumin; Free radical scavenging
Funding
- General Secretariat for Research and Technology (GSRT)
- Hellenic Foundation for Research and Innovation (HFRI), Greek Ministry of Education, Research and Religion
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The interaction of the novel quinazoline (E)-4-(2-(pyridin-2-ylmethylene)hydrazinyl)quinazoline (L) with Zn2+ was performed in the absence or presence of the non-steroidal anti-inflammatory drug sodium diclofenac (Nadicl) and resulted in the formation of complexes [Zn(L)(2)](NO3)(2)center dot MeOH (1-MeOH) and [Zn(L)(diclO)(2)]center dot MeOH (2-MeOH), respectively. The two complexes were characterized by IR and H-1 NMR spectroscopy and by single-crystal X-ray crystallography. In these complexes, L was tridentately coordinated to Zn(II) via the quinazoline, hydrazone and pyridine nitrogen atoms. Further studies concerning the behavior of the compounds towards calf-thymus (CT) DNA and supercoiled circular pBluescript KS II plasmid DNA (pDNA) have been performed. The complexes may bind to CT DNA via intercalation, with complex 1 showing higher binding affinity than 2. The complexes may cleave pDNA in the absence or presence of irradiation with UVA, UVB or visible light and the most active pDNA-cleavager is compound 1. The binding constants of the compounds for bovine serum albumin were calculated and the subdomain of the albumin where the compounds prefer to bind was determined. The free radical scavenging ability of the compounds was evaluated towards 1,1-diphenylpicrylhydrazyl and 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) radicals with complex 2 being the most active compound. Thus, complex of type 1 maybe a lead compound for the development of novel DNA-binders and DNA-cleavers or photo-cleavers for medical and biotechnological on demand applications, whereas the structure of complex type 2 may provide novel antioxidants and radical scavengers.
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