Journal
JOURNAL OF INDUSTRIAL AND ENGINEERING CHEMISTRY
Volume 89, Issue -, Pages 442-447Publisher
ELSEVIER SCIENCE INC
DOI: 10.1016/j.jiec.2020.06.018
Keywords
Calcium-binding nanoprobe; Alendronate; Copper-free click chemistry; Intracellular calcium detection; Bone tissue imaging
Funding
- National Research Foundation of Korea [NRF-2018R1A2B3002001, 2019M3A9E2066883]
- National Research Foundation of Korea [2019M3A9E2066883] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
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We report a self-assembled calcium-binding nanoprobe which emits a near-infrared fluorescence (NIRF) signal. We have designed a calcium-binding NIRF nanoprobe by chemically coupling alendronate (ALN), a specific targeting ligand, and NIRF-emitting Cy5.5-labeled dibenzocyclooctyne, to the two respective ends of azide-polyethylene glycol-NHS ester. Prepared ALN-PEG-Cy5.5 forms the self-assembled nanostructures and has high affinity for calcium phosphates-containing bone minerals. In addition, ALN-PEG-Cy5.5 can be employed to visualize intracellular calcium ion levels in vitro as well as normal bone tissues in vivo. These results suggest that our calcium-binding NIRF nanoprobe has a promising potential for calcium imaging in vitro and in vivo. (C) 2020 The Korean Society of Industrial and Engineering Chemistry. Published by Elsevier B.V. All rights reserved.
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