Influence of the neonicotinoid insecticide thiamethoxam on soil bacterial community composition and metabolic function

Understanding of neonicotinoid insecticides toxicity on non-target organisms, such as bees, has indirectly promoted their soil treatment use. However, their effect on soil ecosystems haven't fully understood. Here, based on 16S rRNA high-throughput sequencing and metagenomics, the effects of neonicotinoid insecticide thiamethoxam on bacterial communities and metabolic functions in two types of soils were studied. Thiamethoxam treatment significantly affected soil bacterial abundance, reduced microbial diversity, and changed the bacterial community structure in the short term, and the structure soon returned to a stable state. Soil type and time were important factors affecting bacterial community structure. Some plant growth-promoting rhizosphere bacteria (PGPR) including Actinobacteria were found, and their populations were reduced, while pollutant-degrading bacteria including Firmicutes were also found, and their populations were increased. Based on metagenomics analysis, thiamethoxam treatment insignificantly promoted or inhibited multiple metabolic processes, but gene abundance of some key processes significantly changed. Subtypes of 18 biodegradation genes (BDGs) and 5 pesticide degradation genes (PDGs) were identified. Thiamethoxam treatment significantly increased the abundance of BDGs and PDGs, including cytochrome P450. Potential hosts of P450 degradation genes, including the genus Rhodococcus, were discovered. Conclusions of this study will promote safety evaluation and degradation-related research on neonicotinoid insecticides in soil.

Automated summary

This study investigated the effects of the neonicotinoid insecticide thiamethoxam on bacterial communities and metabolic functions in two types of soils. Thiamethoxam treatment significantly affected soil bacterial abundance, reduced microbial diversity, and temporarily altered the bacterial community structure. The study also identified changes in the populations of certain beneficial and pollutant-degrading bacteria, as well as the increased abundance of biodegradation and pesticide degradation genes, including cytochrome P450. The conclusions of this study will contribute to safety evaluations and degradation-related research on neonicotinoid insecticides in soil.