Antioxidant responses in cyanobacterium Microcystis aeruginosa caused by two commonly used UV filters, benzophenone-1 and benzophenone-3, at environmentally relevant concentrations

Benzophenone-type ultraviolet filters (BPs) have recently been recognized as emerging organic contaminants. In the present study, the cyanobacterium Microcystis aeruginosa was exposed to environmentally relevant levels (0.01-1000 mu g L-1) of benzophenone-1 (BP-1) and benzophenone-3 (BP-3) for seven days. A battery of tested endpoints associated with photosynthetic pigments and oxidative stress was employed for a better understanding of the mode of action. The tested cyanobacterium could uptake the two BPs (27.4-54.9%) from culture media. The two BPs were able to inhibit the production of chlorophyll a (chl-a) and promote the accumulation of carotenoids, leading to unaffected chl-a autofluorescence. Slightly increased malondialdehyde (MDA) contents suggested that BP-1 and BP-3 caused moderate oxidative stress. BP-1 stimulated the activities of superoxide dismutase (SOD), glutathione reductase (GR) and glutathione S-transferase (GST) in M. aeruginosa while BP-3 increased the activities of SOD, GST, and glutathione (GSH), showing a concentration- and time-dependent relationship. The activities of other biomarkers, such as catalase (CAT) and glutathione peroxidase (GPx) fluctuated depending on exposure time and concentration. The overall results suggested that the two BPs can trigger moderate oxidative stress in M. aeruginosa and the tested cyanobacterium was capable of alleviating stress by different mechanisms.