Journal
JOURNAL OF HAZARDOUS MATERIALS
Volume 405, Issue -, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.jhazmat.2020.124336
Keywords
Arsenate toxicity tolerance; Cell death; Oxidative stress; Phytochelatins; Signalling; Vacuolar sequestration
Categories
Funding
- King Saud University, Riyadh, Saudi Arabia [RSP-2020/194]
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IAA application significantly reduced arsenic accumulation and oxidative stress markers in eggplant seedlings, protecting roots from cell death. Endogenous H2O2 may have played a crucial role as a downstream signal in the IAA-mediated mitigation of arsenate toxicity in eggplant roots.
The role of indole-3-acetic acid (IAA) and hydrogen peroxide (H2O2) crosstalk in regulating metal stress is still less known. Herein, role of IAA in alleviating arsenate (As-V) toxicity in brinjal seedlings along with its probable relation with endogenous H2O2 was investigated. Arsenate hampered root growth due to greater accumulation of As and decrease in phosphorus uptake that resulted into inhibited photosynthesis and cell death. Further, As-V induced oxidative stress markers and damage to macromolecules (lipids and proteins) due to alterations in redox status of glutathione as a result of inhibition in activity of glutathione synthetase and glutathione reductase. However, application of IAA with As-V improved root growth by significantly declining As accumulation and oxidative stress markers, sequestrating As into vacuoles, and improving redox status of glutathione which collectively protected roots from cell death. Interestingly, addition of diphenylene iodonium (DPI, an inhibitor of NADPH oxidase) further increased As-V toxicity even in the presence of IAA. However, application of H2O2 rescued negative effect of DPI. Overall, the results suggested that in IAA-mediated mitigation of As-V toxicity in brinjal roots, endogenous H2O2 might have acted as a downstream signal.
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