4.4 Article

Screening of potential uranium protein targets in fish ovaries after chronic waterborne exposure: Differences and similarities between roach and zebrafish

Journal

JOURNAL OF ENVIRONMENTAL RADIOACTIVITY
Volume 222, Issue -, Pages -

Publisher

ELSEVIER SCI LTD
DOI: 10.1016/j.jenvrad.2020.106365

Keywords

Uranium; Speciation; Ovary; Biomolecules; Vitellogenin; Zebrafish; Roach

Funding

  1. NEEDS-Environment program

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Concentration of uranium (U), a naturally encountered radioactive element in earth's crust, can be enhanced in freshwater ecosystems (mu g.L-1 - mg.L-1) due to various anthropogenic activities. The consequent aquatic organism exposure to U leads to its accumulation in all organs, particularly in the gonad, and in subcellular fractions (mainly the cytosol); then it is known to affect fish at several biological levels, and more particularly, at a reproduction endpoint, with a decrease in the total number of eggs, spawn events and larvae survival. The understanding of U reprotoxicity requires the fine knowledge of its speciation at molecular level, i.e., its interaction with cytosolic biomolecules. In this study, we focus on the U-protein interactions in gonads. A non-denaturating extraction protocol combined with size exclusion chromatography (SEC) allowed the separation of metal-protein complexes in ovaries of U-contaminated wild roaches before their elemental detection (ICP MS). This enables unprecedented information to be obtained about U distribution in ovaries of autochthonous fish, Rutilus rutilus, which is different in some points from that obtained in the model species, Danio rerio under controlled laboratory conditions at a similar concentration level. Finally, the ability to transpose results from model to autochthonous fish was briefly discussed.

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