Journal
JOURNAL OF ELECTROANALYTICAL CHEMISTRY
Volume 873, Issue -, Pages -Publisher
ELSEVIER SCIENCE SA
DOI: 10.1016/j.jelechem.2020.114400
Keywords
Electrochemical immunosensor; Vitamin D; Gold-platinum; Glutaraldehyde; 3-(aminopropyl)triethoxysilane; Bimetallic nanoparticles
Categories
Funding
- Department of Science and Technology
- Special Assistance Programme (UGC-SAP) [F.4-7/2015/DRS-III (SAP-II)]
- DST-SERB [EEQ/2017/000239]
Ask authors/readers for more resources
We have developed a gold-platinum nanoparticles (Au-Pt NPs) supported on 3-(aminopropyl)triethoxysilane (APTES) modified fluorine tin oxide (FTO) glass electrode as a biosensing platform for the detection of 25-hydroxy vitamin D-3 (25(OH)D-3). The Au-Pt NPs were electro-deposited onto the APTES modified FTO electrode using chronoamperometry at a constant potential of -0.2 V for 350 s. The antibody against vitamin D-3 (Ab-25(OH)D-3) was immobilized covalently via glutaraldehyde cross linking chemistry. The successful immobilization of the matrix was confirmed through characterization techniques like FE-SEM, FT-IR, XRD, XPS, contact angle measurement, electrochemical characterizations such as cyclic voltammetry (CV), differential pulse voltammetry (DPV) and electrochemical impedance spectroscopy (EIS). The DPV technique was employed for the detection of 25(OH)D-3 using ferricyanide/ferrocyanide solution [Fe (CN)(6)](3-/4-) as a redox mediator. The immunosensor presented a detection limit of 0.49 pg/mL with a quick response time of 12 min and an acceptable stability of 10 days, if stored at 4 degrees C. The proposed immunosenor is highly selective and sensitive for 25(OH)D-3 and could be employed for the real-time quantification of vitamin D.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available