Antioxidant, Anti-inflammatory and Biosorption Properties of Starch Nanocrystals In Vitro Study: Cytotoxic and Phytotoxic Evaluation

This study aimed to investigate the antioxidant, anti-inflammatory and biosorption properties of starch nanocrystals (SNC). The characterization of synthesized SNC was done using various analytical techniques like microscopic and spectroscopic analysis. The antioxidant property was determined using DPPH (2,2-diphenyl-1-picrylhydrazyl) assay and metal ion chelating assay. SNC showed the highest scavenging activity of 70.03 +/- 0.74% at 100 mu g/mL concentration. Protein denaturation assay and proteinase inhibitory assay depicted the anti-inflammatory property of SNC. The results revealed that the maximum inhibition activity was found at 100 mu g/mL with 72.71% inhibition. The maximum removal efficiency was found to be 83.42% at pH 2.0 with 0.15 g biosorbent. As the pH increases, biosorption capacity of SNC were reduced from 8.17 to 6.30 mg/g and the efficiency of the dye removal was decreased from 80.95 to 36.01%. The shape of synthesized SNC was spherical nanoplatelets and it shows agglomeration. The Langmuir isotherm model is best suited for the biosorption experiments with the R2 value of 0.986. SNC were subjected to cytotoxic and phytotoxic evaluation. Cell viability and phytotoxic assays proves the non-toxic nature of the SNC.

Automated summary

The study investigated the antioxidant, anti-inflammatory, and biosorption properties of starch nanocrystals (SNC). SNC exhibited high scavenging activity and anti-inflammatory properties at certain concentrations. The biosorption capacity of SNC was shown to decrease as pH increased, with optimal removal efficiency at pH 2.0. The Langmuir isotherm model was found to be suitable for biosorption experiments with SNC, with a high R2 value of 0.986, and SNC showed non-toxic characteristics in cytotoxic and phytotoxic evaluations.