Journal
JOURNAL OF CHROMATOGRAPHY A
Volume 1627, Issue -, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.chroma.2020.461421
Keywords
Ultra-large-volume injection; Nano-LC; Liquid electron ionization (LEI)-tandem mass spectrometry; Water-assisted trap focusing; Pesticides; Microfluidics
Funding
- Syngenta Ltd.
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Herein we present an efficient, column-switching method that relies on a custom-made T-union passive diffusion micromixer to assist water dilution and promote trap solute focusing of a high sample volume dissolved in pure organic solvent using a 0.075 mm i.d. nano-LC column. This method allows injecting 20 mu L (or higher) of sample volume, speeding up the analysis time, with a 400-fold increase of the limits of quantitation for selected compounds. Five pesticides in different media were used as model compounds, and the analyses were carried out with a triple quadrupole mass spectrometer equipped with a Liquid Electron Ionization (LEI) LC-MS interface working in multiple reaction monitoring (MRM) mode. The system microfluidics were investigated using COMSOL modeling software. Robustness of the entire system was evaluated using a post-extraction addition soil extracts with limits of detection values spanning from 0.10 to 0.45 mu g/L. Reproducible results in terms of peak area, peak shape, and retention times were achieved in soil matrix. Repeatability test on peak area variations were lower than 10%. (c) 2020 Elsevier B.V. All rights reserved.
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