Journal
JOURNAL OF BIOPHOTONICS
Volume 14, Issue 2, Pages -Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/jbio.202000359
Keywords
fibroblast; light emitting diode; photobiomodulation; red light; skin fibrosis
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Funding
- National Institute of General Medical Sciences of the National Institutes of Health [K23GM117309]
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High-fluence LED-RL may decrease fibroblast proliferation by inducing G(0)/G(1) arrest, providing insight into the molecular mechanism underlying LED-RL as an anti-fibrotic therapeutic.
Skin fibrosis is a debilitating feature of several systemic and dermatologic diseases. While current treatment options carry significant risk of side effects and recurrence, high-fluence light emitting diode-generated red light (LED-RL) is an alternative therapeutic that is safe, non-invasive, and accessible. We previously demonstrated LED-RL decreases fibroblast proliferation, a key pathogenic component of fibrosis. However, the cellular mechanism by which high fluence LED-RL modulates fibroblast proliferation is unclear. Herein, we explored the effects of high fluence LED-RL on human dermal fibroblast cell cycle progression. We demonstrate that LED-RL at 640 J/cm(2) induced significant arrest of cells in G(0)/G(1) compared to temperature-matched control. This was accompanied by a corresponding increase in expression of checkpoint regulator p53 in irradiated cells. These data demonstrate high fluence LED-RL may exert its anti-proliferative effect on fibroblasts by inducing G(0)/G(1) arrest. Further, this study provides insight into the molecular mechanism underlying LED-RL as an anti-fibrotic therapeutic.
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