Synthesis and spectroscopic characterization of new sulfanilamide-functionalized magnetic nanoparticles, and the usability for carbonic anhydrase purification: is there perspective for 'cancer treatment' application?

This investigation indicated an efficient procedure to purify human carbonic anhydrase II (hCA II) enzyme through sulfanilamide-functionalized (gamma-Fe2O3-CPTES-SA) magnetic nanoparticles (MNPs), where synthesis of Fe(3)O(4)MNPs was carried out using co-precipitation reaction. Next, 3-chloropropyltriethoxysilane (CPTES) was used to modify Fe(3)O(4)nanoparticles and lastly, the surface of the nanoparticles was functionalized with sulfanilamide (SA) as a carbonic anhydrase ligand/inhibitor for binding to hCA II. The characterization of the synthesized nanoparticles was performed using various techniques. These characterization methods revealed that the MNPs were effectively coated with CPTES and SA, and the average diameter of the nanoparticles was approximately 21 nm. The possibility of interaction of gamma-Fe2O3-CPTES-SA nanoparticles with hCA II was studied via multi-spectroscopic techniques. The protein isolated by this single-step procedure had high purity as confirmed by a single band on SDS-PAGE (sodium dodecyl sulfate-polyacrylamide gel electrophoresis) which was also active after purification. By focusing on their drug loading capacity, and increasing their specificity and affinity to target CA-expressing cancer cells, the synthesized MNPs may dramatically impact the treatment of cancer and become suitable for clinical use in the near future. Communicated by Ramaswamy H. Sarma

Automated summary

An efficient procedure was developed to purify human carbonic anhydrase II enzyme using functionalized magnetic nanoparticles, showing high purity and activity after purification. The nanoparticles may have a significant impact in cancer treatment and could potentially be used clinically in the near future.