Journal
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
Volume 109, Issue 7, Pages 1209-1219Publisher
WILEY
DOI: 10.1002/jbm.a.37114
Keywords
3D bioprinting; bioink; cell bioscaffolds; human periodontal ligament stem cells; hydrogels
Funding
- Guangdong Natural Science Funds [2018A030313614]
- Fundamental Research Funds for the Central Universities [21619407 JG2019125]
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Bone tissue is essential for supporting and protecting the human body, with bone defects posing a common source of injury. In clinical practice, 3D bioprinting of scaffolds offers a promising solution for reconstruction challenges. Utilizing hydrogels as biomaterials, this study successfully printed SA/Gel/na-HA bioscaffolds and demonstrated their biocompatibility and osteogenic differentiation capabilities using hPDLSCs, showing great potential for bone defect reconstruction.
Bone tissue plays an important role in supporting and protecting the structure and function of the human body. Bone defects are a common source of injury and there are many reconstruction challenges in clinical practice. However, 3D bioprinting of scaffolds provides a promising solution. Hydrogels have emerged as biomaterials with good biocompatibility and are now widely used as cell-loaded materials for bioprinting. This study involved three steps: First, sodium alginate (SA), gelatin (Gel), and nano-hydroxyapatite (na-HA) were mixed into a hydrogel and its rheological properties assessed to identify the optimum slurry for printing. Second, SA/Gel/na-HA bioscaffolds were printed using 3D bioprinting technology and their physical properties characterized for surface morphology, swelling, and mechanical properties. Finally, human periodontal ligament stem cells (hPDLSCs) were mixed with SA/Gel/na-HA printing slurry to create a bioink to prepare SA/Gel/na-HA/ hPDLSCs cell bioscaffolds. These were tested for biocompatibility and osteogenic differentiation performance using live/dead cell staining, cell adhesion, cell proliferation, and alkaline phosphatase activity. The SA/Gel/na-HA hydrogel exhibited shear-thinning behavior. The equilibrium swelling of the bioscaffold was 125.9%, the compression stress was 0.671 MPa, and the compression elastic modulus was 8.27 MPa. The SA/Gel/na-HA/hPDLSCs cell bioscaffolds caused effective stimulation of cell survival, proliferation, and osteoblast differentiation. Therefore, the SA/Gel/na-HA/hPDLSCs cell bioscaffolds displayed potential as a material for bone defect reconstruction.
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