4.6 Article

Microbial enrichment of blackcurrant press residue with conjugated linoleic and linolenic acids

Journal

JOURNAL OF APPLIED MICROBIOLOGY
Volume 130, Issue 5, Pages 1602-1610

Publisher

OXFORD UNIV PRESS
DOI: 10.1111/jam.14888

Keywords

blackcurrant; conjugated linoleic acid; conjugated linolenic acid; press residue; Propionibacterium freudenreichii

Funding

  1. Business Finland [26001642]

Ask authors/readers for more resources

This study demonstrates that Propionibacterium freudenreichii DSM 20270 can simultaneously enrich blackcurrant press residue with health-beneficial conjugated isomers of LA and alpha-LNA. The microbial isomerization technique can be utilized to enrich lipid-containing plant materials with bioactive compounds, enabling valorization of low value plant-based side streams from the food industry into value-added food ingredients.
Aims The aim of the study was to investigate the isomerization of linoleic (LA) and linolenic acids (LNAs) into their conjugated isomers byPropionibacterium freudenreichiiDSM 20270 and utilize this feature for microbial enrichment of blackcurrant press residue (BCPR) with health-beneficial conjugated fatty acids. Methods and Results First, the ability ofP. freudenreichiito isomerize 0 center dot 4 mg ml(-1)of LA and LNA was studied in lactate growth medium. Free LA and alpha-LNA were efficiently converted into conjugated linoleic (CLA) and alpha-linolenic acid (alpha-CLNA), being the predominant isomersc9,t11-CLA andc9,t11,c15-CLNA, respectively. The bioconversion of alpha-LNA byP. freudenreichiiwas more efficient in terms of formation rate, yield and isomer-specificity. Thereafter, free LA and LNAs obtained from hydrolysed BCPR neutral lipids, by lipolytically active oat flour, were subjected to microbial isomerization in BCPR slurries. In 10% (w/v) slurries, a simultaneous enrichment inc9,t11-CLA andc9,t11,c15-CLNA of up to 0 center dot 51 and 0 center dot 29 mg ml(-1)was observed from starting levels of 0 center dot 96 mg LA ml(-1)and 0 center dot 37 mg alpha-LNA ml(-1)respectively. Conclusions This study shows that growing cultures ofP. freudenreichiiDSM 20270 are able to simultaneously enrich BCPR with health-beneficial conjugated isomers of LA and alpha-LNA. Significance and Impact of the Study This study demonstrates that microbial isomerization technique can be utilized to enrich lipid-containing plant materials with bioactive compounds and thereby enable valorization of low value plant-based side streams from food industry into value-added food ingredients.

Authors

I am an author on this paper
Click your name to claim this paper and add it to your profile.

Reviews

Primary Rating

4.6
Not enough ratings

Secondary Ratings

Novelty
-
Significance
-
Scientific rigor
-
Rate this paper

Recommended

No Data Available
No Data Available