Journal
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
Volume 68, Issue 40, Pages 11144-11150Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acs.jafc.0c01900
Keywords
self-assembling; polylysine; NH2-beta-cyclodextrin; nanoparticles; allicin
Funding
- National Key Research and Development Project of China [2017YFC1600901]
- Key Research and Development Projects of Henan Province [202102310142]
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Most chemotherapeutics are hydrophobic molecules and need to be converted into hydrophilic formulations before administration. To address this issue, a novel cyclodextrin-based nanoparticle was proposed as a versatile carrier for cellular delivery of hydrophobic molecules. First, the effect of the polylysine (PL)/NH2-beta-cyclodextrin (NH2-beta-CD) ratio on particle size and encapsulation efficiency in prepared complexes was investigated. Subsequently, transmission electron microscopy images showed that the sizes of PL/NH2-beta-CD nanoparticles ranging from 10 to 260 nm decreased with the reduction in the PL/NH2-beta-CD ratio, which was completely consistent with the findings of size distributions. At a PL/NH2-beta-CD ratio of 10, the surface charge on the PL/NH2-beta-CD nanoparticle was maximized at (+52.8 mV), and encapsulation efficiency was optimal (47.2%), which revealed a great advantage in delivery of hydrophobic allicin. In addition, the positive charge of PL chains facilitated the cellular uptake of the PL/NH2-beta-CD-DOX by interacting with the negatively charged cell membrane. Conclusively, this study suggests that the combination of allicin and PL/NH2-beta-CD nanoparticles acting on the S and G2/M phases in cell cycle regulation induces apoptosis and exhibits substantial application in killing cancer cells.
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