Physico-chemical characterization of carvacrol loaded zein nanoparticles for enhanced anticancer activity and investigation of molecular interactions between them by molecular docking

Carvacrol (CV), a monoterpene possesses wide range of biological activities but has limited application due to low aqueous solubility and poor bioavailability. To address this issue and enhance bioavailability and efficacy of carvacrol, lecithin stabilized zein nanoparticles were investigated. Precipitation method was used for synthesis of nanoparticles and characterized using various techniques. CV entrapped under optimized parameters has size around 250 nm with -15 mV zeta potential. SEM studies showed nanoparticles with spherical morphology and size in accordance with DLS studies. FTIR, NMR and DSC were used to determine the molecular interaction between CV and lecithin stabilized zein nanoparticles. Molecular docking studies were performed to understand the interaction between protein and drug at molecular level. Our results demonstrated the presence of two active sites within zein, showing strong binding interactions with carvacrol. The encapsulation efficiency of 78% with loading efficiency of 13% was obtained as per HPLC and UV-Vis studies. Cytotoxicity assay indicated that the CV loaded nanoparticles induce cytotoxicity against colon cancer (SW480) cells further confirmed by acridine orange and ethidium bromide dual staining assay. Fluorescent tagged nanoparticles revealed significant cellular uptake of drug. Our results suggest that CV can be conveniently delivered via oral route after incorporating into lecithin stabilized zein nanoparticles and may prove effective for colon cancer treatment.