Journal
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
Volume 21, Issue 17, Pages -Publisher
MDPI
DOI: 10.3390/ijms21176338
Keywords
bacteriophage; phage therapy; Stenotrophomonas; phage genomics; phage receptor
Funding
- Natural Sciences and Engineering Research Council of Canada (NSERC)
- CGS-D award from NSERC
- AIGSS award from Alberta Innovates
- NSERC USRA summer studentship
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The rapid increase in the number of worldwide human infections caused by the extremely antibiotic resistant bacterial pathogenStenotrophomonas maltophiliais cause for concern. An alternative treatment solution in the post-antibiotic era is phage therapy, the use of bacteriophages to selectively kill bacterial pathogens. In this study, the novel bacteriophage AXL3 (vB_SmaS-AXL_3) was isolated from soil and characterized. Host range analysis using a panel of 29 clinicalS. maltophiliaisolates shows successful infection of five isolates and electron microscopy indicates that AXL3 is a member of theSiphoviridaefamily. Complete genome sequencing and analysis reveals a 47.5 kb genome predicted to encode 65 proteins. Functionality testing suggests AXL3 is a virulent phage and results show that AXL3 uses the type IV pilus, a virulence factor on the cell surface, as its receptor across its host range. This research identifies a novel virulent phage and characterization suggests that AXL3 is a promising phage therapy candidate, with future research examining modification through genetic engineering to broaden its host range.
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