Journal
INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE
Volume 46, Issue 5, Pages 1653-1660Publisher
SPANDIDOS PUBL LTD
DOI: 10.3892/ijmm.2020.4723
Keywords
cutaneous squamous cell carcinoma; circular RNA_001937; microRNA-597-3p; Fos-related antigen 2; proliferation
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Circular RNAs (circRNAs) are reported to be aberrantly expressed and perform different functions in numerous types of tumor; however, their expression levels in cutaneous squamous cell carcinoma (CSCC) remain largely unclear. Thus, the purpose of the present study was to investigate the function of circRNA_001937 in CSCC. Differential circRNA expression profiles of CSCC were analyzed using the Arraystar Human circRNAs chip and reverse transcription-quantitative PCR (RT-qPCR); and the effects of circRNA_001937 on cell behavior, in particular its regulation over the microRNA (miRNA)-597-3p/Fos-related antigen 2 (FOSL2) pathway, was investigated using a dual-luciferase reporter assay, and verified using RT-qPCR and western blotting. circRNA_001937 expression levels were significantly increased in CSCC tissues and cell lines compared with the corresponding adjacent tissues and control cells (P<0.05). The genetic silencing of circRNA_ 001937 with small interfering RNA significantly inhibited cell proliferation, and induced cell apoptosis (P < 0.05). circRNA_ 001937 was observed to directly bind to miRNA-597-3p and serve as a sponge, which indirectly increased the expression levels of FOSL2, a miRNA-597-3p target gene. In conclusion, circRNA_001937 expression was increased in CSCC and silencing circRNA_001937 gene expression may inhibit CSCC progression by sponging the miRNA-597-3p/FOSL2 pathway.
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