Extended shelf life milk processing: Effect of simulated cleaning in place on the germination and attachment ofBacillus cereusspores

The effect of simulated cleaning in place (CIP) was determined on the structure, attachment and growth ofBacillus cereusspores isolated from raw milk and biofilms in filler nozzles from extended shelf life (ESL) milk processing lines. Simulated CIP treatment structurally affected >98% ofB. cereusspores, while 0.1% remained intact. Following simulated CIP treatment,B. cereusspores were able to attach to stainless steel coupons and form biofilms.B. cereusspores were capable of germination and growth under refrigerated conditions for more than 28 days. Contamination withB. cereusspores may lead to a reduced shelf life and potentially be a safety risk in ESL milk with a prolonged shelf life.

Automated summary

The study found that simulated cleaning in place had a significant impact on the structure of cereus spores, with most spores being affected but some remained intact. These spores were able to attach and grow after treatment, posing potential risks to the shelf life and safety of ESL milk.