Estimating aboveground and organ biomass of plant canopies across the entire season of rice growth with terrestrial laser scanning

Non-destructive and accurate estimation of crop biomass is crucial for the quantitative diagnosis of growth status and timely prediction of grain yield. As an active remote sensing technique, terrestrial laser scanning (TLS) has become increasingly available in crop monitoring for its advantages in recording structural properties. Some researchers have attempted to use TLS data in the estimation of crop aboveground biomass, but only for part of the growing season. Previous studies rarely investigated the estimation of biomass for individual organs, such as the panicles in rice canopies, which led to the poor understanding of TLS technology in monitoring biomass partitioning among organs. The objective of this study was to investigate the potential of TLS in estimating the biomass for individual organs and aboveground biomass of rice and to examine the feasibility of developing universal models for the entire growing season. The field plots experiments were conducted in 2017 and 2018 and involved different nitrogen (N) rates, planting techniques and rice varieties. Three regression approaches, stepwise multiple linear regression (SMLR), random forest regression (RF) and linear mixed-effects (LME) modeling, were evaluated in estimating biomass with extensive TLS and biomass data collected at multiple phenological stages of rice growth across the entire season. The models were calibrated with the 2017 dataset and validated independently with the 2018 dataset. The results demonstrated that growth stage in LME modeling was selected as the most significant random effect on rice growth among the three candidates, which were rice variety, growth stage and planting technique. The LME models grouped by growth stage exhibited higher validation accuracies for all biomass variables over the entire season to varying degrees than SMLR models and RF models. The most pronounced improvement with a LME model was obtained for panicle biomass, with an increase of 0.74 in R-2 (LME: R-2 = 0.90, SMLR: R-2 = 0.16) and a decrease of 1.15 t/ha in RMSE (LME: RMSE = 0.79 t/ha, SMLR: RMSE = 2.94 t/ha). Compared to SMLR and RF, LME modeling yielded similar estimation accuracies of aboveground biomass for pre-heading stages, but significantly higher accuracies for post-heading stages (LME: R-2 = 0.63, RMSE = 2.27 t/ha; SMLR: R-2 = 0.42, RMSE =2.42 t/ha; RF: R-2 = 0.57, RMSE = 2.80 t/ha). These findings implied that SMLR was only suitable for the estimation of biomass at pre-heading stages and LME modeling performed remarkably well across all growth stages, especially for post-heading. The results suggest coupling TLS with LME modeling is a promising approach to monitoring rice biomass at post-heading stages at high accuracy and to overcoming the saturation of canopy reflectance signals encountered in optical remote sensing. It also has great potential in the monitoring of other crops in cloud-cover conditions and the instantaneous prediction of grain yield any time before harvest.