Journal
INFLAMMATION
Volume 44, Issue 1, Pages 407-420Publisher
SPRINGER/PLENUM PUBLISHERS
DOI: 10.1007/s10753-020-01345-3
Keywords
inflammation; miRNAs; macrophages; adipose tissue stem cells; RNA sequencing
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Funding
- National Natural Science Foundation of China [81501663, 81530064]
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The study investigated the impact of adipose-derived stem cells on the inflammatory response, revealing differential expressions of multiple miRNAs and mRNAs. This may contribute to a better understanding of the anti-inflammatory mechanisms of adipose-derived stem cells.
Severe inflammation can lead to multiple organ dysfunction syndrome, which has high mortality. Adipose-derived stem cells have been shown to affect the inflammatory response of macrophages. However, the molecular mechanism of the anti-inflammatory capacity of adipose-derived stem cells (ADSCs) remains to be understood. In the present study, a macrophage inflammation model was established by LPS, and treated with different volumes of ADSC supernatant. Then, we investigated the key genes in the LPS group and treatment group by RT-PCR, RNA sequencing technology, and bioinformatics analysis. A total of 26 miRNAs and 11,882 mRNAs were differentially expressed between them. The expression of 15 of the miRNAs (9 upregulated and 6 downregulated) was confirmed by RT-PCR. GO and KEGG pathway analyses of the targets of the 9 significantly upregulated miRNAs showed that they were related to immune system process, inflammatory response, lipopolysaccharide, and TNF-alpha, NF-kappa B, Toll-like receptor, and MAPK signaling pathways. Moreover, a miRNA-mRNA network also revealed 8 important genes (Mapkapk2, Sepp1, Cers6, Snn, ZfP568, Ccdc93, Pofut1, Pik3cd). We finally confirmed the expression of these 8 targeted genes by performing the RT-PCR analysis. This study may provide a new understanding of the molecular mechanism of ADSCs in the inflammatory response related to multiple miRNAs and mRNAs.
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