Journal
INDIAN JOURNAL OF MICROBIOLOGY
Volume 61, Issue 1, Pages 96-99Publisher
SPRINGER
DOI: 10.1007/s12088-020-00907-1
Keywords
hdcAgene; Histamine; Fermented fish; Herrings; Real time PCR
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This study directly assessed the presence of the hdcA gene in ready-to-eat surströmming samples from three producers in Sweden for the first time. The average gene abundance of hdcA was significantly higher in samples from producer A compared to producers B and C, indicating a potential load of histamine-producing bacteria in surströmming.
Histamine is a degradation product of the bacterial decarboxylation of the amino acid histidine; such activity is determined by histidine decarboxylase encoded by a gene cluster, carried by some Gram-positive bacteria, that includes thehdcAgene. In this study, the presence of thehdcAgene in ready-to-eatsurstrommingsamples collected from three producers based in Sweden was directly assessed via qPCR analysis for the very first time. Samples from producer A showedhdcAaverage gene abundance of 6.67 +/- 0.13 Log cells/gene copies g(-1); in samples from producer B the average value attested at 5.56 +/- 0.06 Log cells/gene copies g(-1), whereas for samples of producer ChdcAaverage gene abundance attested at 5.30 +/- 0.08 Log cells/gene copies g(-1). ANOVA showed a significantly higher averagehdcAgene copy number in samples from producer A, whereas no significant differences were seen between average values ofhdcAgene copy numbers detected in samples from producer B and C. ThehdcAgene copies detected in the present study could give an estimation of the load of potential histamine-producing bacteria insurstromming.
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