Journal
GERODONTOLOGY
Volume 38, Issue 1, Pages 87-94Publisher
WILEY
DOI: 10.1111/ger.12500
Keywords
biofilms; denture cleansers; denture hygiene; effervescent tablets
Funding
- FAPESP [2013/05494-1]
- CAPES
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The study found that peroxide-based solutions have a certain antimicrobial effect on multispecies biofilms, effectively reducing the viability and metabolic activity of bacteria, but unable to completely remove the biofilm.
Purpose To investigate the impact of peroxide-based solutions in reducing viability and metabolic activity of multispecies biofilms on denture base acrylic resin surfaces and for removing them from these surfaces. Background Denture cleansers are effective in reducing monospecies biofilm; however, studies evaluating their action on multispecies biofilms are scarce. Materials and methods Sixty-nine denture base acrylic resin specimens (o 15 x 3 mm) were sterilised then contaminated withCandida albicans,Staphylococcus aureusandPseudomonas aeruginosato form multispecies biofilms. Biofilms were grown for 24 hours; subsequently, specimens were immersed in three different cleansing solutions (n = 9): nitradine (NI), fixodent (FX) and phosphate-buffered saline (Control), according to the respective manufacturer's instructions. After applying the hygiene protocols, viability of microorganisms was evaluated by counting colony-forming units and assessing metabolic activity. Moreover, biofilm removal capacity was estimated based on extension of cell-covered areas visualised in fluorescent microscopy micrographics. Results Microbial counts were solution-dependent; NI was effective against all microorganisms (P < .05). FX exhibited moderate antimicrobial action, reducingP aeruginosa(P < .05) andS aureus(P < .05) viability by approximately 2 logs. Both peroxide-based solutions reduced metabolic activity (P < .001) and biofilm-covered areas on specimen surfaces (P < .001). Conclusion Under the experimental conditions tested, these results demonstrated that peroxide-based solutions had favourable antimicrobial activity but promoted no broad elimination of aggregated multispecies biofilm. NI might be more suitable as complementary chemical agent for controlling multispecies denture biofilm.
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