Journal
GENOMICS
Volume 113, Issue 1, Pages 450-461Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ygeno.2020.09.009
Keywords
Osteosarcoma; Long-non-coding RNA-ROR; microRNA-185-3p; Yes-associated protein 1; Proliferation; Apoptosis; Migration; Invasion
Funding
- National Natural Science Foundation of China [81502331]
- Wisdom Accumulation and Talent Cultivation Project of The Third Xiangya Hosipital of Central South University
- Natural Science Foundation of Hunan Province [2018SK2090]
- National Key Research and Development Program of China [2016YFC1201800]
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This study demonstrates that down-regulation of lncRNA-ROR or up-regulation of miR-185-3p inhibits osteosarcoma progression through the repression of YAP1.
Aim: The co-expression network of long non-coding RNA ROR (lncRNA-ROR) and microRNA-185-3p (miR-1853p) has not been focused on osteosarcoma. Therein, this work was initiated to uncover lncRNA-ROR and miR185-3p functions in osteosarcoma. Methods: LncRNA-ROR, miR-185-3p and Yes-associated protein 1 (YAP1) expression in osteosarcoma tissues and cells were detected. The screened cells (MG63 and U2OS) were transfected with decreased and/or increased lncRNA-ROR and miR-185-3p to explore osteosarcoma progression. Tumor growth was detected by tumor xenografts in mice. Results: Up-regulated lncRNA-ROR and YAP1 and down-regulated miR-185-3p were found in osteosarcoma. LncRNA ROR knockdown or miR-185-3p overexpression inhibited osteosarcoma cell progression while lncRNA ROR elevation or miR-185-3p inhibition presented the opposite effects. Function of lncRNA ROR was rescued by miR-185-3p and regulated the growth and metastasis of osteosarcoma cells via modulating YAP1, the target gene of miR-185-3p. Conclusion: This work illustrates that lncRNA-ROR down-regulation or miR-185-3p up-regulation inhibits osteosarcoma progression via YAP1 repression.
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