4.6 Article

Exonuclease 5 is dispensable for meiotic progression and male fertility in mouse

Journal

GENE
Volume 769, Issue -, Pages -

Publisher

ELSEVIER
DOI: 10.1016/j.gene.2020.145254

Keywords

Exo5; Spermatogenesis; Fertility; Mismatch repair

Funding

  1. National Key Research and Developmental Program of China [2018YFC1003403, 2016YFC1000600]
  2. National Natural Science Foundation of China [31890780, 31630050]
  3. Strategic Priority Research Program of the Chinese Academy of Sciences [XDB19000000]
  4. Fundamental Research Funds for the Central Universities [YD2070002006]

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The study found that Exo5 knockout mice are fertile despite a slight decrease in sperm count, and showed no remarkable differences in epididymal histology and testis/body weight ratio. Under normal breeding conditions, Exo5 seems to have no essential role in spermatogenesis in mice.
Exonuclease 5 (Exo5) belongs to a class of bi-directional, ssDNA-specific exonucleases that mainly involved in the DNA repair pathways. Exo5 has been reported to be crucial for DNADNA mismatch repair (MMR) in several human cell lines. However, its in vivo function in mammals still needs to be explored. Thus, to study the in vivo role of Exo5 in spermatogenesis, Exo5 knockout mice were generated using CRISPR/Cas9 technology. Unexpectedly, we found that the knockout mice are fertile despite a slight decrease in sperm count. Furthermore, Exo5(-/-) mice showed no detectable developmental anomalies, exhibited no remarkable differences in the epididymal histology and testis/body weight ratio. Moreover, cytological investigations on meiocytes revealed non-significant differences in chromosomal synapsis, recombination, and meiotic progression of prophase I, further demonstrating that Exo5 has no essential role in spermatogenesis in mice under normal breeding conditions. Collectively, these data indicate that Exo5 is dispensable for meiotic progression and fertility in mice.

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