Journal
EXPERIMENTAL CELL RESEARCH
Volume 394, Issue 2, Pages -Publisher
ELSEVIER INC
DOI: 10.1016/j.yexcr.2020.112150
Keywords
Taste organoid; Circumvallate papillae; Lgr5; Age; Car4; Gustducin; Signaling pathway
Categories
Funding
- National Natural Science Foundation of China [81700894, 31771155, 31900714]
- Shanghai Municipal Education Commission
- Shanghai Eastern Scholar Program [TP2016029]
- Shanghai Municipal Human Resources and Social Security Bureau, Shanghai Talent Development Fund [2018112]
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Sense of taste is central to evaluate food before digestion. Taste stem cells undergo constant differentiation throughout the life. However, the mechanism underlying the generation of taste receptor cells is still not clear. Here, we cultured taste organoids from either Lgr5 + or Lgr5-cells, and found the preferential generation of Car4 + and Gustducin + taste receptor cells in organoids derived from Lgr5 + cells in circumvallate, foliate or fungiform papillae. Taste organoids derived from Lgr5 + cells in circumvallate papillae of neonatal mice showed stronger capacity to generate taste receptor cells compared to the organoids from Lgr5 + cells of the adult circumvallate papillae. Massive transcriptional differences were found in multiple signaling pathways including taste transduction between organoids derived from circumvallate papillae of adult and neonatal mice. Inhibiting the Notch signaling pathway by LY411575 enhanced taste receptor cell generation in organoids from circumvallate papillae and modulated multiple signaling pathways. Thus, we concluded that receptor cell generation in taste organoids was age-related and regulated via multiple signaling pathways.
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