Journal
EMBO JOURNAL
Volume 39, Issue 20, Pages -Publisher
WILEY
DOI: 10.15252/embj.2019103667
Keywords
DNAmethylation; fluorescence-activated nuclei sorting; plant development; stem cells; transcriptome
Categories
Funding
- GMI/IMP/IMBA Biooptics facilities
- Next Generation Sequencing and Plant Sciences units of the Vienna BioCenter Core Facilities (VBCF)
- Austrian Science Fund [FWF I489, I1477, I3687]
- Cost Action [16212]
- Plant Fellows program (EU FP7)
- Austrian Science Fund (FWF) [I3687, I1477] Funding Source: Austrian Science Fund (FWF)
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In plants, aerial organs originate continuously from stem cells in the center of the shoot apical meristem. Descendants of stem cells in the subepidermal layer are progenitors of germ cells, giving rise to male and female gametes. In these cells, mutations, including insertions of transposable elements or viruses, must be avoided to preserve genome integrity across generations. To investigate the molecular characteristics of stem cells inArabidopsis, we isolated their nuclei and analyzed stage-specific gene expression andDNAmethylation in plants of different ages. Stem cell expression signatures are largely defined by developmental stage but include a core set of stem cell-specific genes, among which are genes implicated in epigenetic silencing. Transiently increased expression of transposable elements in meristems prior to flower induction correlates with increasingCHGmethylation during development and decreasedCHHmethylation, before stem cells enter the reproductive lineage. These results suggest that epigenetic reprogramming may occur at an early stage in this lineage and could contribute to genome protection in stem cells during germline development.
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