4.5 Article

An Electrochemical ATP Biosensor with Enzymes Entrapped within a PEDOT Film

Journal

ELECTROANALYSIS
Volume 33, Issue 2, Pages 1-12

Publisher

WILEY-V C H VERLAG GMBH
DOI: 10.1002/elan.202060397

Keywords

adenosine triphosphate (ATP); polyethylenedioxythiophene (PEDOT); glucose oxidase and hexokinase; electrochemical biosensor

Funding

  1. National Institutes of Health [R01 DK094932]
  2. Innovation Discretionary Funding Initiative (DFI) Award through the MSU Office of the Senior Vice President for Research and Innovation

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The ATP biosensor described in this paper utilizes glucose oxidase and hexokinase immobilized within the electroactive polymer PEDOT, achieving indirect detection of ATP at different detection potentials with good detection performance.
This paper reports on the electrochemical behavior of an ATP biosensor that utilizes glucose oxidase (GOx) and hexokinase (Hex) immobilized within the electroactive polymer, polyethylenedioxythiophene (PEDOT). This biosensor design detects ATP indirectly at 0.85 V vs. Ag/AgCl based on the oxidation current for enzymatically generated H2O2, and at -0.20 V; a potential at which improved analyte selectivity is achieved. The detection figures of merit at both detection potentials are a response time of 15 +/- 1 s, an experimental detection limit of 10.0 +/- 0.2 mu mol L-1(S/N=3), and a sensitivity in the range of 100-500 mA M(-1)cm(-2).

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