Journal
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
Volume 98, Issue 2, Pages -Publisher
ELSEVIER SCIENCE INC
DOI: 10.1016/j.diagmicrobio.2020.115120
Keywords
Rapid; Carbapenemases; Laboratory detection; Enterobacterales
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Funding
- Calgary Laboratory Services [10015169]
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Purpose: The rapid detection of carbapenemases among Enterobacterales in clinical laboratories is critical for management of patients, and infection prevention and control efforts. Methods: A study was designed to evaluate the performances of the RAPIDEC CARBA NP (R), beta-CARBA (R), NG-Test CARBA 5 (R), modified carbapenem-inactivation method, and EDTA version (eCIM) assays against a global collection of Enterobacterales (n = 216) with diverse carbapenemases. Results: The RAPIDEC CARBA NP (R) assay had a sensitivity of 98.68 and specificity of 19.6% and beta-CARBA (R) a sensitivity of 94% and specificity of 97.8%, but showed low sensitivity with Klebsiella Pneumoniae Carbapenemase (KPC)-containing isolates. The NG-Test CARBA 5 (R) had an overall sensitivity of 96.3% and specificity of 100% and failed to detect isolates with bla(IMP-13), bla(IMP-14). The eCIM gave false- positive results with Oxacillinase (OXA)-48-like enzymes. Conclusion: The NG-Test CARBA 5 (R) assay was technically simple and provided rapid accurate results on the types of carbapenemases. Such information has potential treatment benefits for patients. (C) 2020 Elsevier Inc All rights reserved.
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