Journal
CYTOSKELETON
Volume 77, Issue 10, Pages 379-398Publisher
WILEY
DOI: 10.1002/cm.21633
Keywords
actin; C; elegans; cytoskeleton; filamin; LINC complex; myosin
Categories
Funding
- PNational Science Foundation [1816640]
- NIH NIA [R01G044346, R00AG052666]
- National Institutes of Health
- National Center for Research Resources
- Div Of Molecular and Cellular Bioscience
- Direct For Biological Sciences [1816640] Funding Source: National Science Foundation
- NATIONAL INSTITUTE ON AGING [R01AG044346] Funding Source: NIH RePORTER
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Actomyosin networks are organized in space, direction, size, and connectivity to produce coordinated contractions across cells. We use theC. elegansspermatheca, a tube composed of contractile myoepithelial cells, to study how actomyosin structures are organized. FLN-1/filamin is required for the formation and stabilization of a regular array of parallel, contractile, actomyosin fibers in this tissue. Loss offln-1results in the detachment of actin fibers from the basal surface, which then accumulate along the cell junctions and are stabilized by spectrin. In addition, actin and myosin are captured at the nucleus by the linker of nucleoskeleton and cytoskeleton complex (LINC) complex, where they form large foci. Nuclear positioning and morphology, distribution of the endoplasmic reticulum and the mitochondrial network are also disrupted. These results demonstrate that filamin is required to prevent large actin bundle formation and detachment, to prevent excess nuclear localization of actin and myosin, and to ensure correct positioning of organelles.
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