Journal
CURRENT OPINION IN BIOTECHNOLOGY
Volume 65, Issue -, Pages 156-162Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.copbio.2020.02.015
Keywords
-
Funding
- US National Science Foundation through the Division of Molecular and Cellular Biosciences [MCB-1817708]
Ask authors/readers for more resources
Metabolic engineering seeks to reprogram cells to efficiently produce value-added chemicals. Traditionally, this is achieved by overexpressing the production pathway and/or knocking out competing endogenous pathways. However, limitations in some pathways are more effectively addressed through dynamic metabolic flux control to favor different cellular objectives over the course of the fermentation. Dynamic control circuits can autonomously actuate changes in metabolic fluxes in response to changing fermentation conditions, cell density, or metabolite concentrations. In this review, we discuss recent studies focused on multiplexed autonomous strategies which (1) combine regulatory circuits to control metabolic flux at multiple nodes or (2) respond to more than one input signal. These strategies have the potential to address challenging pathway scenarios, actuate more complex response profiles, and improve the specificity of the criteria that actuate the dynamic response.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available