Journal
CRITICAL REVIEWS IN BIOTECHNOLOGY
Volume 41, Issue 1, Pages 1-15Publisher
TAYLOR & FRANCIS LTD
DOI: 10.1080/07388551.2020.1825320
Keywords
Endo-beta-mannanase; over-expression; functional dissection; enzyme stability; substrate hydrolysis; rational designing; directed evolution
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Funding
- UGC, Govt. of India
- CSIR-CFTRI, Mysuru [MLP 0116]
- CSIR, New Delhi [BSC0112]
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This review provides a comprehensive overview of research on endo-beta-mannanase engineering, covering key factors such as activity stability, substrate recognition, and mode of action, as well as rational and directed evolution approaches to improve industrial biocatalysts.
The hydrolysis of mannans by endo-beta-mannanases continues to gather significance as exemplified by its commercial applications in food, feed, and a rekindled interest in biorefineries. The present review provides a comprehensive account of fundamental research and fascinating insights in the field of endo-beta-mannanase engineering in order to improve over-expression and to decipher molecular determinants governing activity-stability during harsh conditions, substrate recognition, polysaccharide specificity, endo/exo mode of action and multi-functional activities in the modular polypeptide. In-depth analysis of the available literature has also been made on rational and directed evolution approaches, which have translated native endo-beta-mannanases into superior biocatalysts for satisfying industrial requirements.
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