Journal
COLLOIDS AND SURFACES B-BIOINTERFACES
Volume 194, Issue -, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.colsurfb.2020.111187
Keywords
Membrane biophysical models; Cellular membranes; Lipid vesicles; Lipophilicity; Membrane fluidity; Membrane location
Funding
- Laboratory for Process Engineering, Environment, Biotechnology and Energy - LEPABE - national funds through the FCT/MCTES (PIDDAC) [UIDB/00511/2020]
- European Union's Horizon 2020 research and innovation programme [810685]
- Santa Casa da Misericordia de Lisboa [MB-37-2017]
- FCT [SFRH/BD/129312/2017]
- FCT - Fundacao para a Ciencia e a Tecnologia, I.P., under the Scientific Employment Stimulus - Institutional Call [CEECINST/00049/2018]
- Fundação para a Ciência e a Tecnologia [SFRH/BD/129312/2017] Funding Source: FCT
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Vitamin B12 (VB12) deficiency is one of the most common malnutrition problems worldwide and is related to its poor bioavailability. The lipid composition of cell membranes and molecule-cell membrane lipid interactions are major factors affecting the bioavailability of nutrients. So, the study of these interactions may allow predicting the behavior of VB12 at cellular membranes and the effects on its activity. Thus, lipid vesicles with lipid composition similar to the majority of eukaryotic cell membranes were used as biomembrane models, and their interactions with VB12 molecules were evaluated. For that, different parameters were assessed such as the lipophilicity of VB12, its preferential location in the membrane and its effect on the physical properties of the bilayer. VB12 showed high affinity for the biological membranes, not inducing any biophysical changes in their properties. The interactions of VB12 with the membrane was affected by the complexity of the bilayer, since its increase in order and rigidity hinders the diffusion of molecules. Thus, the low bioavailability of VB12 is not related with its interactions with the biological membranes.
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