Journal
CLINICAL MICROBIOLOGY AND INFECTION
Volume 27, Issue 2, Pages -Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.cmi.2020.09.057
Keywords
Antigen detection; Diagnosis; Fluorescence immunochromatographic assay; Nucleocapsid protein; SARS-CoV-2
Categories
Funding
- National Key R&D Program of China [2016YFA0502204]
- National Natural Science Foundation of China [82041007, 81925026]
- Chongqing Health Commission COVID-19 Project [2020ZX01]
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The FIC assay for NP antigen testing shows high specificity and relatively high sensitivity in early-phase SARS-CoV-2 diagnosis, according to a study conducted in Wuhan and Chongqing between February 10 and 15, 2020.
Objectives: Rapid, reliable and easy-to-implement diagnostics that can be adapted in early severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) diagnosis are critical to combat the epidemic. SARS-CoV-2 nucleocapsid protein (NP) is an ideal target for viral antigen-based detection. A rapid and convenient method was developed based on fluorescence immunochromatographic (FIC) assay to detect the SARS-CoV-2 NP antigen. However, the accuracy of this diagnostic method needs to be examined. Methods: This prospective study was carried out between 10 and 15 February 2020 in seven hospitals in Wuhan and one hospital in Chongqing, China. Participants with clinically suspected SARS-CoV-2 infection were enrolled. NP antigen testing by FIC assay and nucleic acid (NA) testing by real-time reverse transcriptase PCR (RT-PCR) were performed simultaneously in a blinded manner with the same nasopharyngeal swab sample. The diagnostic accuracy of NP antigen testing was calculated by taking NA testing of RT-PCR as the reference standard, in which samples with a cycle threshold (C-r) value of <= 40 were interpreted as positive for SARS-CoV-2. Results: A total of 253 participants were enrolled; two participants were excluded from the analyses because of invalid NP testing results. Of 251 participants (99.2%) included in the diagnostic accuracy analysis, 201 (80.1%) had a C-r value of <= 40. With C-r value 40 as the cutoff of NA testing, the sensitivity, specificity and percentage agreement of the FIC assay was 75.6% (95% confidence interval, 69.0-81.3), 100% (95% confidence interval, 91.1-100) and 80.5% (95% confidence interval, 75.1-84.9) respectively. Conclusions: With RT-PCR assay as the reference standard, NP antigen testing by FIC assay shows high specificity and relatively high sensitivity in SARS-CoV-2 diagnosis in the early phase of infection. (C) 2020 European Society of Clinical Microbiology and Infectious Diseases. Published by Elsevier Ltd. All rights reserved.
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