Journal
CIRCULATION JOURNAL
Volume 84, Issue 11, Pages 2027-2031Publisher
JAPANESE CIRCULATION SOC
DOI: 10.1253/circj.CJ-20-0881
Keywords
COVID-19; Myocarditis; SARS-CoV-2; Stem cells
Categories
Funding
- Theme-based Research Scheme, University Grant Committee [T11-707/15-R]
- University Development Fund, The University of Hong Kong, Hong Kong SAR, China
- Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Ministry of Education, China
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Background: SARS-CoV-2 infection is associated with myocardial injury, but there is a paucity of experimental platforms for the condition. Methods and Results: Human-induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) infected by SARS-CoV-2 for 3 days ceased beating and exhibited cytopathogenic changes with reduced viability. Active viral replication was evidenced by an increase in supernatant SARS-CoV-2 and the presence of SARS-CoV-2 nucleocaspid protein within hiPSC-CMs. Expressions of BNP, CXCL1, CXCL2, IL-6, IL-8 and TNF-alpha were upregulated, while ACE2 was downregulated. Conclusions: Our hiPSC-CM-based in-vitro SARS-CoV-2 myocarditis model recapitulated the cytopathogenic effects and cytokine/chemokine response. It could be exploited as a drug screening platform.
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