Journal
CHEMOSPHERE
Volume 255, Issue -, Pages -Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.chemosphere.2020.126914
Keywords
Polystyrene microplastics; Freshwater microalgae; Euglena gracilis; Oxidative stress; Gene expression analysis
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Funding
- National Major Project of Science & Technology Ministry of China [2017ZX0730100203, 2017ZX07204004-04]
- National Natural Science Foundation of China [41773115, 41571468]
- Science and Technology Support Program of Jiangsu Province [BE2016736]
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Microplastics have aroused widespread concern because of their adverse effects on aquatic organisms. However, the underlying toxicity mechanisms have not been examined in detail. This study investigated the interactions between polystyrene microplastics (PS-MPs) and the model freshwater microalgae Euglena gracilis. The results of transmission electron microscopy showed that the vacuoles of microalgae were induced after 24 h exposure to 1 mg/L PS-MPs (5 mu m and 0.1 mu m). Furthermore, PS-MPs significantly (p < 0.05) reduced pigment contents. Moreover, superoxide dismutase activities were significantly (p < 0.05) induced in all PS-MPs treated groups. Peroxidase activities were also significantly (p < 0.05) affected by two sizes of PS-MPs (5 mu m and 0.1 mu m), indicating that oxidative stress was induced after exposure to PS-MPs. At the molecular level, PS-MPs dysregulated the expression of genes involved in cellular processes, genetic information processing, organismal systems, and metabolisms. The KCS gene and the CTR1 gene may be key pathways to induce adverse effects on the E. gracilis after exposure to 5 tm PS-MPs. These findings will help to elucidate the underlying molecular mechanism of microplastics toxicity on freshwater organisms. (C) 2020 Elsevier Ltd. All rights reserved.
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