Journal
CHEMICAL REVIEWS
Volume 121, Issue 9, Pages 5098-5123Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acs.chemrev.0c00587
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Funding
- National Institutes of Health [R01-AI081059, R01-AI153358, R01GM100951]
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The asymmetric lipid bilayer of Gram-negative bacteria's outer membrane is crucial for antibiotic resistance, assembled by specific protein machines such as the Lpt system and Mla pathway.
The outer membrane of Gram-negative bacteria is essential for their survival in harsh environments and provides intrinsic resistance to many antibiotics. This membrane is remarkable; it is a highly asymmetric lipid bilayer. The inner leaflet of the outer membrane contains phospholipids, whereas the fatty acyl chains attached to lipopolysaccharide (LPS) comprise the hydrophobic portion of the outer leaflet. This lipid asymmetry, and in particular the exclusion of phospholipids from the outer leaflet, is key to creating an almost impenetrable barrier to hydrophobic molecules that can otherwise pass through phospholipid bilayers. It has long been known that these lipids are not made in the outer membrane. It is now believed that conserved multisubunit protein machines extract these lipids after their synthesis is completed at the inner membrane and transport them to the outer membrane. A longstanding question is how the cell builds and maintains this asymmetric lipid bilayer in coordination with the assembly of the other components of the cell envelope. This Review describes the transenvelope lipid transport systems that have been identified to participate in outer-membrane biogenesis: LPS transport via the Lpt machine, and phospholipid transport via the Mla pathway and several recently proposed transporters.
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