Journal
CARBOHYDRATE POLYMERS
Volume 245, Issue -, Pages -Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.carbpol.2020.116500
Keywords
FucoPol; Polysaccharide; Cryopreservation; Calorimetry; Cytotoxicity; Non-colligative
Categories
Funding
- UCIBIO - FCT/MCTES [UIDB/04378/2020]
- LAQV - FCT/MCTES [UID/QUI/50006/2020]
- CENIMAT/I3N - FCT/MCTES [UIDB/50025/2020]
- [SFRH/BD/144258/2019]
- Fundação para a Ciência e a Tecnologia [SFRH/BD/144258/2019] Funding Source: FCT
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We report the cryoprotective potential of FucoPol, a fucose-containing bacterial exopolysaccharide produced by Enterobacter A47. In vitro cryopreservation assays of Vero, Saos-2, HFFF2 and C2C12 cell lines exposed to a validated non-cytotoxic 2.5 mg/mL FucoPol concentration demonstrated a consistent post-thaw metabolic viability increase. Calorimetric analysis showed a non-colligative, FucoPol concentration-dependent increase of the freezing point (T-f), with minimal change in melting point (T-m). Freezing point variation was corroborated by Polarized Optical Microscopy studies, also showing a reduction of ice crystal dimensions. Its proven shear -thinning behaviour and polyanionicity favour interactivity between the polysaccharide and the water-ice in-terface, resulting in ice growth inhibition. These findings demonstrate FucoPol's high promise as a bio-based, biodegradable approach to be implemented into cryopreservation formulations.
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