Journal
BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY
Volume 68, Issue 6, Pages 1113-1119Publisher
WILEY
DOI: 10.1002/bab.2030
Keywords
biosensors; enzyme; immobilization; pesticide; polypyrrole
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A voltammetric biosensor for detecting ATCh and paraoxon was successfully developed with good storage stability and operational stability. The linear range for ATCh was 30-50 mu M and 0.46-1.84 nM for paraoxon, with detection limits of 0.45 mu M and 0.17 nM, respectively.
A voltammetric biosensor for acetylthiocholine (ATCh) and paraoxon detection was successfully developed. To achieve this goal, polypyrrole (PPy) was synthesized onto the platinum (Pt) electrode surface in 0.30 M oxalic acid solution containing 25 mM pyrrole. PPy-coated Pt (Pt/PPy) electrode surface was covered with chitosan (Chi) (Pt/PPy/Chi). The acetylcholinesterase (AChE) enzyme was immobilized on the Pt/PPy/Chi electrode surface to build a voltammetric biosensor (Pt/PPy/Chi/AChE). The storage stability of the biosensor was determined to be 72% even after 60 days. The operational stability was determined to be 94% after 20 consecutive measurements. For the biosensor, the linear range was determined to be 30-50 mu M for ATCh and 0.46-1.84 nM for paraoxon. The limit of detection (LOD) was determined to be 0.45 mu M for ATCh and 0.17 nM for paraoxon
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