Journal
BIOSENSORS & BIOELECTRONICS
Volume 171, Issue -, Pages -Publisher
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2020.112753
Keywords
Lateral flow sensor; Gold nanoparticle; Structure-directing agents; Copper in-situ growth; Signal amplification; Infectious disease
Categories
Funding
- National Key Research and Development Program of China [2018YFC1602203, 2018YFC1602202]
- National Natural Science Foundation of China [32001788, 31760485, 31901780]
- Interdisciplinary Innovation Fund of Natural Science, Nanchang University [9166-27060003-ZD01]
- Jiangxi Provincial Natural Science Foundation [20202ACB215004]
- Opening Fund of Jiangsu Key Laboratory for Food Quality
- First Affiliated Hospital of Nanchang University (Nanchang, China)
- SafetyState Key Laboratory Cultivation Base, Ministry of Science and Technology [028074911709]
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The PEI-CISG technology introduced in this study controls the growth of copper nanoshells on the surface of gold nanoparticles in AuNP-LFS, effectively enhancing the detection sensitivity for various infectious diseases.
A polyethyleneimine (PEI)-assisted copper in-situ growth (CISG) strategy was proposed as a controlled signal amplification strategy to enhance the sensitivity of gold nanoparticle-based lateral flow sensors (AuNP-LFS). The controlled signal amplification is achieved by introducing PEI as a structure-directing agent to regulate the thermodynamics of anisotropic Cu nanoshell growth on the AuNP surface, thus controlling shape and size of the resultant AuNP@Cu core-shell nanostructures and confining free reduction and self-nucleation of Cu2+ for improved reproducibility and decreased false positives. The PEI-CISG-enhanced AuNP-LFS showed ultrahigh sensitivities with the detection limits of 50 fg mL(-1) for HIV-1 capsid p24 antigen and 6 CFU mL(-1) for Escherichia coli O157:H7. We further demonstrated its clinical diagnostic efficacy by configuring PEI-CISG into a commercial AuNP-LFS detection kit for SARS-CoV-2 antibody detection. Altogether, this work provides a reliable signal amplification platform to dramatically enhance the sensitivity of AuNP-LFS for rapid and accurate diagnostics of various infectious diseases.
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